PCR

PCR

 


The polymerase chain reaction (PCR) is a biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.



PCR mixture
1. dNTPs (A,T,C,G)
2. primer 
3. Template (DNA Target)
4. Polymerase
5. Buffer
6. Thermal cycler machine (PCR machine)

PCR Process step
1. Deneturetion
2. Annealing
3. Extension







PCR ppt download

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